Abstract
The voltage-dependent motor protein Prestin (SLC26A5) is responsible for the electromotive behavior of outer hair cells and underlies the cochlear amplifier1. Knock out or impairment of Prestin causes severe hearing loss2–5. Despite Prestin’s key role in hearing, the mechanism by which mammalian Prestin senses voltage and transduces it into cellular-scale movements (electromotility) is poorly understood. Here, we determined the structure of dolphin Prestin in six distinct states using single particle cryo-electron microscopy. Our structural and functional data suggest that Prestin adopts a unique and complex set of states, tunable by the identity of bound anions (Cl- or SO42-). Salicylate, a drug that can cause reversible hearing loss, competes for the anion-binding site of Prestin, and inhibits its function by immobilizing Prestin in a novel conformation. Our data suggests that the bound anion together with its coordinating charged residues and helical dipole act as a dynamic voltage sensor. Analysis of all anion-dependent conformations reveals how structural rearrangements in the voltage sensor are coupled to conformational transitions at the protein-membrane interface, suggesting a novel mechanism of area expansion. Visualization of Prestin’s electromotility cycle distinguishes Prestin from closely related SLC26 anion transporters, highlighting the basis for evolutionary specialization of the mammalian cochlear amplifier at high resolution.
Access options
Subscribe to Journal
Get full journal access for 1 year
199,00 €
only 3,90 € per issue
Tax calculation will be finalised during checkout.
Rent or Buy article
Get time limited or full article access on ReadCube.
from$8.99
All prices are NET prices.
Author information
Affiliations
Corresponding author
Supplementary information
Supplementary Information
This file contains further supportive results for the findings in this study. Supplementary information includes figures 1 to 10 (including the uncropped gel source data).
Supplementary Video 1
Electromotility measurements of HEK 293 cells transfected with dolphin Prestin using whole-cell patch clamp electrophysiology. To evoke Prestin-mediated electromotility, the membrane potential was held at -70 mV; a 10-mV increase-in-amplitude voltage steps were applied up to the final steps, which was from +150 mV to -140 mV (Fig. 1b). The magenta square indicates the area that was chosen in our custom-written code to track the cellular displacements.
Supplementary Video 2
Structural changes from the Expanded (Down I) to the Compact (Up) conformation as a linear interpolation. The side front and top views of the dimer have been shown in one single frame. The anion-binding site has been highlighted in red and R399 has been shown in Stick representation and the backbone has been colored in yellow. The movies are made in UCSF ChimeraX.
Rights and permissions
About this article
Cite this article
Bavi, N., Clark, M.D., Contreras, G.F. et al. Prestin’s conformational cycle underlies outer hair cell electromotility. Nature (2021). https://ift.tt/3Ea24gi
-
Received:
-
Accepted:
-
Published:
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
"cycle" - Google News
October 25, 2021 at 10:51PM
https://ift.tt/3ntjEoJ
Prestin's conformational cycle underlies outer hair cell electromotility - Nature.com
"cycle" - Google News
https://ift.tt/32MWqxP
https://ift.tt/3b0YXrX
Bagikan Berita Ini
0 Response to "Prestin's conformational cycle underlies outer hair cell electromotility - Nature.com"
Post a Comment